Showing posts with label how to. Show all posts
Showing posts with label how to. Show all posts

Wednesday, July 14, 2010

How to prepare phosphate buffer solution (PBS) for microfluidics use?

Buffer solutions are very important in microfluidics use. In this post I will share my experience on how to prepare PBS. I have been using 100mM phosphate buffer of pH 7.4.

Chemicals Required: Sodium mono phoaphate (NaH2 PO4 H2O, FW=138), sodium diphosphate (Na2HPO4 , FW=142). For the molecular weight check the container of the chemicals.
Procedure: